Global Profiling

Metabolomics, a comprehensive analysis of small molecular compounds in biological samples, is a powerful tool because almost all biological phenomena result in changes in an organismal or cellular metabolic state. However, grasping global changes in metabolism is not easy because it requires the precise measurement of these compounds with a variety of physical and chemical properties.

Global Profiling

We provide a broad profiling of hydrophilic metabolites with our Capillary Electrophoresis Mass Spectrometric (CE-MS) platforms, adding additional hydrophobic metabolites with our LC-MS platform to capture a wide variety of metabolites and pathways.

Basic Scan / Dual Scan – Multipurpose Profiling for Global Metabolism

Global metabolic profiling targets more than 1,200 metabolites involved in primary and secondary metabolism pathways shared by many organisms.

Our unique CE-MS analysis provides accurate and robust profiling including the metabolism of sugars, amino acids, nucleotides, lipids, and stress response biomarkers. Together with CE-MS, LC-MS based lipidomics provides changes in lipid turnover engaged in cellular energy and signaling pathways.

This package is useful for researchers in a variety of areas and sample species.

  • Global measurement of > 1,200 metabolites involved in primary and secondary metabolism Basic Scan: CE-MS analysis of sugars, amino acids, nucleotides and other ionic metabolites Dual Scan: LC-MS analysis of fatty acids, steroids and other lipids in addition to Basic Scan
  • Report including pathway mapping, PCA, HCA and statistical analysis
  • Suitable for a variety of research areas including medical, agriculture, bioprocessing, etc.

Advanced Scan – Ultimate Metabolomics for Biomarker Discovery

Red blood cells carry oxygen to all body tissues, Erythrocytes background, 3d illustration

Our Advanced profiling mode covers the complete metabolome including unknown metabolites observed in CE-TOFMS data analysis. In addition to cell derived metabolites, peptides, exogenous compounds, and disease specific novel compounds will be detected and screened as biomarkers for disease or bioprocessing.

All projects are performed under QC conditions based on established SOPs and experience of over 10 years of operation. Together with multivariate and statistical analysis, this package is the ultimate tool for biomarker screening and metabolism based profiling.

  • Profiling of whole compounds measured by CE-MS including unknown metabolites
  • Unknown compounds accompany precise m/z assisting the prediction of chemical structure
  • Report including pathway mapping, PCA, HCA, statistical analysis and follow up discussion
  • Suitable for biomarker screening and metabolome based profiling

Mediator Scan – Targeted for Signaling and the Inflammasome

In contrast to the LC-MS method for Dual Scan, M-SCAN represents a novel targeted method for measuring lipid mediators, critical signaling modulators involved in many pathways such as inflammation, allergic response, oxidative stress, and the various metabolic diseases.

The sample protocol for M-SCAN also minimizes large triglycerides in favor of 400 lipid mediators. While the current protocol is designed for relative expression, quantitative profiling for selected lipids can be requested by our clients.

M-Scan Targets Include:

  • Fatty acid-derived – prostaglandins, thromboxanes, leukotrienes, lipoxins etc.
  • Phosphate structures – platelet activating factors, endogenous cannabinoids (anandamide, 2-acylglycerol), lysophosphatidic acids, lysophotidylcholines, lysophosphatidylserines, sphingosine-1-phosphates etc.
  • Sterol structures – glucocorticoids (glucose corticoid), aldosterones (mineral corticoid), sex steroids (estrogen, androgen etc.), bile acids, vitamin D, etc.

Lipid mediators have a wide variety of effects on physiological functions, including immunity, biological defense, blood-pressure regulation, pain or fever, gastrointestinal tract activity and growth, division, and differential regulation of cells. Many diseases are associated with failure in functional balance of such effects. A large portion of lipid mediators act by binding with G-protein coupled receptors found on the cell membrane. Meanwhile, steroid hormones, vitamin A, vitamin D, and others bind with nuclear receptors and induce gene expression changes.

Optional Analysis

  • Absolute quantitation of 110 target metabolites for CE-MS analysis PDF
  • LC-MS analysis for lipidomics
  • CE-MS analysis for short peptides
  • Additional data analysis (PCA, HCA, pathway mapping etc.)
  • Feasibility experimental test for sample assessment
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