Applications

At HMT we provide a collaborative atmosphere working with our clients to find solutions by utilizing the maximum potential of our current platforms and working with our clients to drive new technology development at HMT.

One of the most significant hallmarks of cancer is its dependence on high levels of glucose and an impaired respiratory capacity despite the presence of oxygen, often termed the Warburg Effect. While most cancers do rely heavily on glucose supply, recent years has shown that cancer cells maintain a high level of metabolic flexibility.

Isotopomer analyses have become a crucial next step for researchers with steady state metabolomics data. These analyses rely on the use of heavy atom labeled substrates such as C13 glucose. With the sensitivity of the capillary electrophoresis separation, we can accurately resolve the various isotopomers of the labeled metabolites.

Metabolomics as an omics is gaining traction among the basic sciences. It is becoming more and more evident that genetic changes affecting protein production and activity has a direct relationship to metabolite levels and function. Studying metabolites and their changes are providing insights that simple gene changes cannot predict. Metabolomics is the phenotypic manifestation of genetic and protein changes.

HMT employs advanced CE-MS and LC-MS technologies to analyze a wide range of metabolites. Our platforms are tailored to capture data on signaling molecules, polyamines, and energy metabolism from diverse sample types, including brain and nervous tissues, biofluids, retinal samples, and neuronal cells.

Metabolomics comprehensively characterizes small polar and lipid metabolites, yielding a snapshot of physiological processes that vary according to the pathological state of cells, tissues, and organs. Therefore, the use of metabolomics can help to reveal specific metabolic alterations and potential biomarkers that are associated with disease severity in patients with systemic infections.

The field of skin metabolism is growing as we try to understand the dynamics of the largest organ in our body and first line of defense for our bodies.

Our project scientists will work with you to create cellular metabolism pathway maps unique to your study to enable understanding the metabolism of your cell type.

Bacterial cells, Fungi extracts, plants, algae represent a group of specialty samples and HMT has analyzed with protocols to optimize polar and lipid extractions. Cell extractions, cell pellets, plant roots and leaves, algae extracts and algae cells can be analyzed using HMT protocols.

An essential requirement for food products and future foods is taste. The perception of taste encompasses many senses – textures, odors, visual and taste receptors. With HMT profiling we employ both CE and LC methods including peptides to deliver a tastant profile.

At HMT we use a 4-point profiling platform using OMEGA Scan, Q353, LC-OMEGA and MSCAN to product an annotated metabolite list for human plasma consisting of over 1200 polar and lipid metabolites in a data set to monitor common plasma based polar and lipid metabolites. Each platform is designed and optimized for specific classes of metabolites. Such a large metabolome allows us to capture human biodiversity in healthy and disease populations.

At HMT we have several platforms including C-SCOPE, F-SCOPE and OMEGA Scan to measure and quantitate mitochondrial pathways. Our project scientists will help construct a pathway map unique to your goals based upon our comprehensive analysis, literature and our collaboration.

At HMT, our capillary electrophoresis separation methods provide the highest separation method that is capable of providing highest degree of metabolite specificity and diversity. Our 4 point analysis provides options for discovery: OMEGA Scan, LC-OMEGA, Q353 and MSCAN. These options may provide over 1200 annotated metabolites in human plasma.

HMT’s CEMS based platform maximizes polar metabolite coverage, while our LCMS platform covers an extensive list of lipids, fatty acids, bile acids and acyl carnitines. Our project scientists will help construct pathway maps unique to your project goals to enable to address cell growth and product profiles.